Immunogold electron-microscopic localisation of calpain I in skeletal muscle of rats
N Yoshimura, T Murachi, R Heath, J Kay, B Jasani… - Cell and tissue …, 1986 - Springer
N Yoshimura, T Murachi, R Heath, J Kay, B Jasani, GR Newman
Cell and tissue research, 1986•SpringerBy using a double-affmity-purified first antibody and colloidal gold-conjugated second
antibody, it is shown that calpain I (a cysteine proteinase activated by micromolar
concentrations of Ca 2+) has a predominant intracellular location in the I-band region of the
extensor digitorum longus (EDL) muscle of the rat, but is not exclusively associated with the
Z-line.
antibody, it is shown that calpain I (a cysteine proteinase activated by micromolar
concentrations of Ca 2+) has a predominant intracellular location in the I-band region of the
extensor digitorum longus (EDL) muscle of the rat, but is not exclusively associated with the
Z-line.
Summary
By using a double-affmity-purified first antibody and colloidal gold-conjugated second antibody, it is shown that calpain I (a cysteine proteinase activated by micromolar concentrations of Ca2+) has a predominant intracellular location in the I-band region of the extensor digitorum longus (EDL) muscle of the rat, but is not exclusively associated with the Z-line.
Springer