[HTML][HTML] An experimental strategy to probe Gq contribution to signal transduction in living cells
J Patt, J Alenfelder, EM Pfeil, JH Voss, N Merten… - Journal of Biological …, 2021 - ASBMB
Heterotrimeric G protein subunits Gαq and Gα11 are inhibited by two cyclic depsipeptides,
FR900359 (FR) and YM-254890 (YM), both of which are being used widely to implicate
Gq/11 proteins in the regulation of diverse biological processes. An emerging major
research question therefore is whether the cellular effects of both inhibitors are on-target,
that is, mediated via specific inhibition of Gq/11 proteins, or off-target, that is, the result of
nonspecific interactions with other proteins. Here we introduce a versatile experimental …
FR900359 (FR) and YM-254890 (YM), both of which are being used widely to implicate
Gq/11 proteins in the regulation of diverse biological processes. An emerging major
research question therefore is whether the cellular effects of both inhibitors are on-target,
that is, mediated via specific inhibition of Gq/11 proteins, or off-target, that is, the result of
nonspecific interactions with other proteins. Here we introduce a versatile experimental …
