Reduced neutrophil elastase inhibitor elafin and elevated transforming growth factor-β1 are linked to inflammatory response in sputum of cystic fibrosis patients with …
JC Thomassen, T Trojan, M Walz, C Vohlen… - ERJ Open …, 2021 - Eur Respiratory Soc
Research question Pulmonary disease progression in patients with cystic fibrosis (CF) is
characterised by inflammation and fibrosis and aggravated by Pseudomonas aeruginosa
(Pa). We investigated the impact of Pa specifically on: 1) protease/antiprotease balance; 2)
inflammation; and 3) the link of both parameters to clinical parameters of CF patients.
Methods Transforming growth factor-β1 (TGF-β1), interleukin (IL)-1β, IL-8, neutrophil
elastase (NE) and elastase inhibitor elafin were measured (ELISA assays), and gene …
characterised by inflammation and fibrosis and aggravated by Pseudomonas aeruginosa
(Pa). We investigated the impact of Pa specifically on: 1) protease/antiprotease balance; 2)
inflammation; and 3) the link of both parameters to clinical parameters of CF patients.
Methods Transforming growth factor-β1 (TGF-β1), interleukin (IL)-1β, IL-8, neutrophil
elastase (NE) and elastase inhibitor elafin were measured (ELISA assays), and gene …
Reduced neutrophil elastase inhibitor elafin and elevated TGFβ1 are linked to inflammatory response in sputum of CF patients with Pseudomonas aeruginosa
JC Thomassen, T Trojan, M Walz, C Vohlen… - ERJ Open …, 2021 - Eur Respiratory Soc
Research question Pulmonary disease progression in patients with cystic fibrosis (CF) is
characterized by inflammation and fibrosis, and aggravated by Pseudomonas aeruginosa
(Pa). We investigated the impact of Pa specifically 1) on protease/antiprotease balance and
2) inflammation, as well as 3) the link of both parameters to clinical parameters of CF-
patients. Methods TGFβ 1, IL1β, IL8, neutrophil elastase (NE) and elastase inhibitor elafin
were measured (ELISA assays), and gene expression of the NF-ĸB pathway was assessed …
characterized by inflammation and fibrosis, and aggravated by Pseudomonas aeruginosa
(Pa). We investigated the impact of Pa specifically 1) on protease/antiprotease balance and
2) inflammation, as well as 3) the link of both parameters to clinical parameters of CF-
patients. Methods TGFβ 1, IL1β, IL8, neutrophil elastase (NE) and elastase inhibitor elafin
were measured (ELISA assays), and gene expression of the NF-ĸB pathway was assessed …