[HTML][HTML] The exosome secretory pathway transports amyloid precursor protein carboxyl-terminal fragments from the cell into the brain extracellular space
R Perez-Gonzalez, SA Gauthier, A Kumar… - Journal of Biological …, 2012 - ASBMB
In vitro studies have shown that neuronal cell cultures secrete exosomes containing amyloid-
β precursor protein (APP) and the APP-processing products, C-terminal fragments (CTFs)
and amyloid-β (Aβ). We investigated the secretion of full-length APP (flAPP) and APP CTFs
via the exosome secretory pathway in vivo. To this end, we developed a novel protocol
designed to isolate exosomes secreted into mouse brain extracellular space. Exosomes with
typical morphology were isolated from freshly removed mouse brains and from frozen …
β precursor protein (APP) and the APP-processing products, C-terminal fragments (CTFs)
and amyloid-β (Aβ). We investigated the secretion of full-length APP (flAPP) and APP CTFs
via the exosome secretory pathway in vivo. To this end, we developed a novel protocol
designed to isolate exosomes secreted into mouse brain extracellular space. Exosomes with
typical morphology were isolated from freshly removed mouse brains and from frozen …