[PDF][PDF] REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS
C Anagnostopoulos, J Spizizen - Journal of bacteriology, 1961 - Am Soc Microbiol
C Anagnostopoulos, J Spizizen
Journal of bacteriology, 1961•Am Soc MicrobiolMATERIALS AND METHODS Isolation of deoxyribonucleic acid (DNA). DNA was isolated
from wild-type organisms, such as B. subtilis strain 23 or from prototrophic trans-formants.
Organisms were grown for 12 to 16 hr at 37 C in flasks ona rotary shaker in a medium
containing 1% lactate, 0.25% yeast extract
from wild-type organisms, such as B. subtilis strain 23 or from prototrophic trans-formants.
Organisms were grown for 12 to 16 hr at 37 C in flasks ona rotary shaker in a medium
containing 1% lactate, 0.25% yeast extract
MATERIALS AND METHODS
Isolation of deoxyribonucleic acid (DNA). DNA was isolated from wild-type organisms, such as B. subtilis strain 23 or from prototrophic trans-formants. Organisms were grown for 12 to 16 hr at 37 C in flasks ona rotary shaker in a medium containing 1% lactate, 0.25% yeast extract
American Society for Microbiology