[HTML][HTML] MiaB protein is a bifunctional radical-S-adenosylmethionine enzyme involved in thiolation and methylation of tRNA
The last biosynthetic step for 2-methylthio-N 6-isopentenyl-adenosine (ms 2 i 6 A), present at
position 37 in some tRNAs, consists of the methylthiolation of the isopentenyl-adenosine (i 6
A) precursor. In this work we have reconstituted in vitro the conversion of i 6 A to ms 2 i 6 A
within a tRNA substrate using the iron-sulfur MiaB protein, S-adenosylmethionine (AdoMet),
and a reducing agent. We show that a synthetic i 6 A-containing RNA corresponding to the
anticodon stem loop of tRNA Phe is also a substrate. This study demonstrates that MiaB …
position 37 in some tRNAs, consists of the methylthiolation of the isopentenyl-adenosine (i 6
A) precursor. In this work we have reconstituted in vitro the conversion of i 6 A to ms 2 i 6 A
within a tRNA substrate using the iron-sulfur MiaB protein, S-adenosylmethionine (AdoMet),
and a reducing agent. We show that a synthetic i 6 A-containing RNA corresponding to the
anticodon stem loop of tRNA Phe is also a substrate. This study demonstrates that MiaB …
