Simultaneous three-color analysis of the surface phenotype and DNA-RNA quantitation using 7-amino-actinomycin D and pyronin Y
K Toba, EF Winton, T Koike, A Shibata - Journal of immunological methods, 1995 - Elsevier
We developed an improved technique that permits simultaneous DNA and RNA quantitation
by a flow cytofluorometry using 7-amino-actinomycin D (7AAD) and pyronin Y (PY),
respectively. Detailed cell cycle analyses based upon the cellular DNA/RNA levels were
performed using cells suspended in a buffer containing 0.004% saponin. This method
preserved the light scattering properties of human peripheral blood cells, thus lymphocyte,
monocyte and granulocyte populations could be evaluated. In addition, since 7AAD and PY …
by a flow cytofluorometry using 7-amino-actinomycin D (7AAD) and pyronin Y (PY),
respectively. Detailed cell cycle analyses based upon the cellular DNA/RNA levels were
performed using cells suspended in a buffer containing 0.004% saponin. This method
preserved the light scattering properties of human peripheral blood cells, thus lymphocyte,
monocyte and granulocyte populations could be evaluated. In addition, since 7AAD and PY …
